Modification of Viral Synthesis in Tissue Culture by Substituting Pyruvate For Glucose in the Medium. ∗

Abstract

Summary

Chorioallantoic membrane tissue cultures (CamTC) in balanced salt solution (BSS) with pyruvate as the sole carbon source supported synthesis of influenza virus (strain WS) but not its neurotropic variant (strain NWS). Various other substrates were studied alone and together with pyruvate for their effect on synthesis of strain NWS in chorioallantoic membrane. Cells maintained in BSS containing glucose, xylose, or glycerol as the sole carbon source supported viral synthesis, while cells maintained in ribose, dihydroxyacetone, glycerophosphate, adenosine-5 phosphate, adenosine diphosphate, adenosine triphosphate, or diphosphopyridine nucleotide supported viral synthesis only in combination with pyruvate. It is suggested that synthesis of strain NWS in CamTC requires nutritional factors which are not supplied by cells maintained in a medium with pyruvate as the sole carbon source.