Abstract
Summary
The combination of 0.1% yeast extract and 0.1% proteose peptone #3 in Hanks'balanced salt solution plus 20% horse serum stimulated the rate of growth and produced a higher total count of L cells at the end of 5 days than did the standard reference medium (60% medium 199, 20% whole egg ultrafiltrate, and 20% horse serum). Lowering the serum content in the new medium to 10% still permitted better growth than the standard reference medium. The yeast extract-proteose peptone solution could be sterilized by autoclaving without affecting its growth-stimulating properties.
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