Abstract
Summary
A method has been devised for preparation of highly purified pancreatic callicrein. The fractionation procedure involves three ethanolic fractionations at pH 5.5, 7.5. and 6.5 followed by one ammonium sulfate precipitation. Solutions of hypotensive enzyme at this level of purity proved highly unstable and further purification was abandoned. Preliminary purification of urinary callicrein revealed that the method could be adapted to this source.
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