Abstract
Summary
The cytopathogenic action of the poliovirus strains Akron. Brooks, and Mabie on either tube or Petri plate cultures of monkey kidney cells is considerably delayed when cystine is omitted from the maintenance medium. Omission of all the vitamins or of any one of the 12 other amino acids except glutamine normally present in the maintenance medium resulted in no delay in the appearance of the cytopathogenic effect of Akron PP1. Omission of glutamine resulted in a short delay or no delay. It has further been shown that the propagation of Akron is slower in the absence of cystine. The lowest concentration of L-cystine which will give optimal or near-optimal cytopaithogenic action by Akron is about 10-4 M. Glutathione and L-cysteine hydrochloride can replace cystine at about the same concentration; DL-homocysteine thiolactone hydrochloride and DL-cystathionine can replace also but only at higher molar concentrations. None of 13 other compounds was found to replace cystine. The adsorption of Akron onto monkey kidney cells is not affected by the absence of cystine.
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