Abstract
Summary
The agitated culture has been used in studying the growth cycle of strain L (Earle) mouse fibroblasts. From data available, it is seen that mammalian cells growing in vitro display a growth pattern quite similar to that of other microbiological populations. Some points which have been studied and can now be evaluated are the following: 1) the lag period for these cells appeared to be eliminated completely when log-phase cells were used as inoculum in media equilibrated to pH 7,2) growth rate appeared to be independent of the inoculum size and also independent of serum concentration, 3) generation time for these connective tissue cells. under conditions studied, was approximately 40 hours: 2 generations could usually be observed in these cultures and saturation concentration in some instances reached 2,000.000 cells per ml, 4) steady-state cultivation of tissue cells has been approached. The results indicated that populations could be maintained in such a manner, and this problem will be investigated.
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