Abstract
Summary
1. Microbiological assay procedures were found to be applicable to the determination of free amino acids in depro-teinized plasma but only in the absence of interfering substances. The apparent (relative) values obtained in the presence of interfering substances were shown to be meaningful but only when determined over a wide range of concentration of plasma filtrates. 2. It was concluded that the concentration of some free amino acids (histidine, isoleucine, leucine, ly-sine, phenylalanine. proline, serine and val-ine) was not significantly different but that of some apparent amino acids was higher (ar-ginine and glycine) and others (methionine and threonine) lower in the deproteinized plasma of uremic (nephrectomized) compared to normal dogs.
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