Abstract
Summary
(1) The effect of salt concentration and pH during extraction of sheep pituitary tissue, and temperature and pH of fractionation of these extracts on the preparation of FSH were studied. The most highly purified FSH from the biological standpoint was obtained by extracting at pH 5 with saturated NaCl solution and fractionating at pH 2.5 and 30°C. (2) The FSH preparations made by extracting with 90% saturated and saturated NaCl at pH 5, 5.5 and 6 and fractionating at 30°C and pH 2.5 contained little LH. (3) These preparations were free of ACTH and TSH at the levels tested. (4) The FSH can be concentrated and purified by use of anion and cation exchange resins. (5) Preparations made from 80% saturated NaCl extracts and from extracts made at pH 8 and 9 with saturated NaCl contained significant amounts of LH.
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