Abstract
Summary
1. Methods of concentrating soluble proteins are reported for aqueous extracts of cornea. These methods apparently do not denature the proteins. 2. These methods were applied to increase concentration of protein from aqueous extracts of bovine, swine, New Zealand White rabbit and mongrel rabbit corneas to a level which would permit ultracentrifugal analysis of these extracts. 3. Fundamental differences in number of protein fractions and particle size distribution were noted in these extracts. This suggests a species difference in soluble corneal proteins. 4. Differences were noted in sedimentation diagrams of extracts from New Zealand White rabbits and mongrel rabbits, suggesting differences in soluble corneal protein content of different strains of the same species.
The authors wish to thank Lt. Col. L. J. Milch of the School of Aviation Medicine, Randolph Field, Texas for valuable advice and evaluation of this work.
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