Topical Localization in Mouse of Radioactive Silver Oxide [(Ag 111 ) 2 O] Introduced by Various Routes. ∗

Abstract

Summary and Conclusions

(1) Radioactive silver (Ag¹¹¹) was injected in the form of the insoluble oxide into subcutaneous tissue and serous cavities of normal and tumor bearing (Sarcoma 180, Carcinoma C₃H/BA, Sarcoma McGhee, Carcinoma EO771) mice, in doses of 0.1 or 0.2 millicurie. Twenty-four hours later the animals were sacrificed; in each series some mice were used for autoradiography and others for testing radioactivity of their tissues and organs with Geiger counter. (2) Subcutaneously injected (Ag¹¹¹)₂O is stored in normal mice mainly in the injected area and in smaller proportion by liver and spleen; in subcutaneous tumor bearing mice, the major portion of silver oxide injected in vicinity of the malignant growth is taken up by malignant tissue. (3) (Ag¹¹¹)₂O injected into the normal pleural cavity is stored mainly by lungs and liver, and in tumor bearing cavity—by malignant exudate and implants. Similar difference in distribution was recorded for normal and tumor bearing peritoneal cavities. (4) It was concluded that radioactive (Ag¹¹¹)₂O deposited subcutaneously or inside serous cavities is drained by tissue or exudate fluid into adjacent malignant tissue where it is stored by intercellular fluid and by macrophagic elements.