Abstract
Summary
A new, specific method for hydroxyproline is reported, based on the development of a characteristic chromogen through ninhydrin development of paper chromatograms followed by rapid treatment with dilute alkali and dilute acid. The new chromogen has an absorption maximum at 550 to 560 mμ and fluoresces strongly under ultraviolet light. Application of the method to complex biological media showed that 5 to 10 μg of hydroxyproline could be detected. A high level of this amino acid was shown in chick embryo extract for the first time.
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