Abstract
Summary
A method has been presented for the rapid determination of arginase with a minimum of operations and equipment. The activity of as low as 20 γ of whole liver can be determined. The arginine remaining after enzymatic hydrolysis is estimated by a quantitative modification(9) of the Sakaguchi reaction. Additional studies are presented indicating the necessary substrate concentration, the pH optimum, and linearity of the enzyme concentration curve for these experimental conditions.
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