In vitro Metabolism of DL-AIanine-2-C-14 and Glycine-2-G-14 by Trichinella spiralis Larvae. ∗

Abstract

Summary

1. Trichinella spiralis larvae incorporated C¹⁴ when cultured in vitro in a serum-Rrebs-Ringer medium or Rrebs-Ringer medium containing either dl-alanine-2-C-14 or glycine-2-C-14. 2. Carbon-14 analysis of the larvae revealed a progressive uptake of the C¹⁴-labeled amino acids in both types of media through 48 hours of incubation. 3. The larvae incorporated more C¹⁴ activity (cpm) from glycine-2-C-14-labeled media than from dl-alanine-2-C-14-labeled media. 4. Of the total C¹⁴ incorporated by larvae cultured 48 hours in glycine-2-C-14-la-beled media about 70 to 84% was precipitable by tungstic acid, and was presumably chiefly in the proteins. Larvae cultured in the dl-alanine-2-C-14-labeled media incorporated about 32-57% of their total C14 content into material precipitable by tungstic acid.