Abstract
Conclusions
1. The sulfhydryl reagent, p-chloromercuribenzoic acid, causes hemolysis of human erythrocytes. This action can be prevented by prior reaction with sulfhydryl groups. 2. The hemolytic action of PCMB is reversible by washing cells for as long as 30 minutes after contact with this agent. Reversibility of hemolysis lasts up to 60 minutes after addition of free sulfhydryl group. 3. No protection from PCMB hemolysis was noted with stroma whereas hemoglobin solution was effective. 4. The reaction between PCMB and the -SH group of erythrocytes is relatively slow. 5. A physiologic concentration of glucose offered no protection from hemolysis in this system.
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