Abstract
Summary
1. A preparation from Mycobacterium phlei with decarboxylase activity towards L-glutamic acid only, was described. The preparation has no detectable decarboxylase activity towards other 18 amino-acids tested, and no glutaminase or glutamo-racemase activities. This enables direct estimation of L-glutamic acid in natural products. 2. The optimal pH range is between pH 5.2-5.4, with a sharp drop in activity at pH values of 4 and 6 respectively. 3. Some purification of the enzyme was achieved by fractionation with ammonium sulphate.
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