Vesicular Stomatitis Virus in Tissue Cultures and Cell Suspensions

Abstract

Summary

Vesicular stomatitis virus, New Jersey type, was propagated in suspensions of surviving trypsin-dispersed kidney cells of guinea pigs as well as in cultivated epithelial cells and fibrocytes. Both production methods gave nearly equal virus yields, the highest being approximately 10 6 7 8 9 tissue culture ID₅₀ ml. The highest virus concentration in cultures and suspensions were most frequently recorded at 24 and 72 hours following infection, respectively. Cell suspensions were increased in volume from 2 ml to 200 ml without altering the per ml virus yields. After peak virus concentrations had been reached, the rate of destruction of virus infectivity upon further incubation was significantly higher for cultures than for suspensions. Vesicular stomatitis virus was also shown to grow in suspensions of dispersed bovine kidney cells.