Abstract
Summary
A procedure has been detailed for a 1000-fold purification of a bacterial hyaluronidase from CI. perfringens, strain 2278. Initial crude fractionation with ammonium sulphate followed by three ethanol fractionations at pH 9.0, 6.8 and 7.5 respectively have yielded hyaluronidase fractions containing 200000 TRU/mg N with recovery of 45% of the crude enzyme.
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