Abstract
Summary
1. A method is described for determination of Evans blue dye in various animal tissues. In brief it consists of (1) homo-genization of tissue in a concentrated solution of urea, (2) splitting of the Evans blue-protein complex and partial precipitation of protein and chromogens with acetone, and (3) complete precipitation of chromogens by addition of Somogyi reagents. 2. The method has been employed to follow the fate of the dye after intravenous injection into rats. After such administration of 12.5 mg of Evans blue, about 90% of the injected dye apparently left the blood but only about 15% could not be recovered from the body in 40 hours. At 140 hours after injection about 67% of the injected dye could still be recovered from the body.
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