Abstract
Summary
Plasma accelerator globulin, antihemophilic factor as well as serum eluate were found to produce increased thrombin elaboration in a reaction mixture consisting of bovine plasma eluate, platelets and calcium ions. In the presence of both plasma accelerator globulin and antihemophilic factor, conversion of prothrombin to thrombin became accelerated. Addition of serum eluate to the plasma eluate enhanced the accelerating influence of either plasma accelerator globulin, of antihemophilic factor or of both in the utilization of prothrombin. Upon preincubation of those factors which are supposed to participate in the conversion of prothrombin, before addition of a plasma eluate to the incubation mixture, it was found that more prothrombin was converted in a shorter period of time than in the one-stage thrombin formation procedure. The greater velocitv of thrombin formation in the two-stage procedure indicated that precursory interactions of certain factors had taken place during the initial incubation. Evidence has been obtained that antihemophilic factor as well as plasma accelerator globulin will interact with platelets and calcium ions only in the presence of factor(s) in serum or plasma eluate yielding prothrombin converting activity.
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