Abstract
Summary
1. Human bone marrow of a patient with sickle cell anemia and peripheral blood of a normal individual were incubated with iron-59 under similar conditions. Auto-radiograms were prepared from the cells. After incubation, hemolysates of the cells were fractionated by paper electrophoresis and the radioactivity of the fractions was measured. 2. 90% of the total radioactivity was located in hemoglobin from bone marrow hemoly sates. At 37°C the rate of hemoglobin synthesis as measured by iron-5 9 utilization is more rapid in the most immature erythrocytic cells beginning with the rubriblast. The rate of synthesis at 0°C was 25% of that at 37°C. 3. Less than 5% of the radioactive iron in incubated normal peripheral blood was in the hemoglobin fraction.
Get full access to this article
View all access options for this article.