Abstract
Summary
1. Growth of human and animal synovial membrane in tissue cultures showed characteristic differences from control cultures of periarticular tissues. 2. Presence of hyaluronic acid in about two-thirds of supernates of tissue cultures from synovium was demonstrated by precipitation reactions with acetic acid, enzymatic reactions, viscosity determination, tests for metachromasia and electrophoretic patterns. 3. Quantitative determinations by a turbidimetric method yielded a concentration of hyaluronic acid from 4 to 15 mg %. 4. Tests were negative for sulfonated mucopolysaccharides such as chondroitin sulfuric acid or heparin. 5. Control cultures of periarticular connective tissue were negative for hyaluronic acid and sulfonated mucopolysaccharides.
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