Studies on I 131 Red Blood Cell Antibodies

Abstract

Summary

1. Antisera to sheep and to human red cells were tagged with I¹³¹ and the proteins separated by starch electrophoresis. 2. By measuring the amount of radioactivity bound to the specific antigenic red cell, antibody activity of each fraction of serum was determined. 3. Sheep red cells adsorbed most of the radioactivity from a gamma globulin fraction of rabbit antiserum (hemagglutinin). 4. Type “O” human red cells adsorbed most of the I¹³¹ protein from a gamma globulin fraction of acquired hemolytic anemia serum (auto-antibody).