Abstract
Summary
1. Two methods, one using free fluorescein and the other protein bound fluorescein, were employed to test the capillary anastomosis between rats united in parabiosis. Free fluorescein was demonstrable by galvanometer readings on the abdominal skin of the recipient in fewer than half of the experiments. Protein bound fluorescein, on the other hand, was uniformly detected in the heart blood of both donor and recipient over a period ranging from 4 1/2 to 24 1/2 hours. Precipitation tests were also positive in demonstrating the presence of protein bound fluorescein in heart blood. 2. Parabionts tested by either method showed no adverse effects from the treatment and were in good condition for subsequent investigations. By testing a group of parabionts with protein bound fluorescein one could determine the adequacy of the parabiotic union prior to further experimentation.
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