Abstract
Summary
Release of spermatozoa by the frog, Rana pipiens, after administration of “purified” FSH, LH, prolactin and HCG has been studied. The minimal doses of these hormones which produced a response in over 75% of the animals in each group were: 5 mg of FSH, 0.05 mg of LH and 25 LIT. of HCG. Prolactin did not elicit a response. Our data indicate that the release of spermatozoa in the frog can be induced by LH or a substance with an LH-like activity (HCG). The positive response to FSH is attributed by us to the contamination of the preparation with LH (2 to 3% by manufacturer's assay). It is suggested that this rapid and sensitive response to LH might be utilized to assay the purity of FSH preparations.
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