Abstract
Summary
Preparation of elementary bodies of meningo-pneumonitis virus for electron microscopy by freeze-drying provided spherical particles of 250 to 270 mμ diameter instead of the flattened particles of 380 to 435 mμ diameter obtained by mounting and air drying the specimen in the usual manner. The diameters of the frozen-dried particles corresponded well with diameters estimated by filtration and centrifugation of elementary bodies in the suspended state. Thus, the freeze-dry method preserved approximately the form and dimensions which the elementary bodies possessed in the hydrated state and probably in the infected cell.
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