Abstract
Summary
The osazone method for ascorbic analysis(l) and a number of published modifications of this method (2-4) have been evaluated to derive a procedure which is simultaneously sensitive, specific, accurate, and easy to carry out for routine analyses. In the resulting method, the time-consuming use of Norit and 85% sulfuric acid(l) is avoided, the sensitivity is twice that obtained when glacial acetic acid (3) is used, and a source of error introduced with the use of 2,6-dichloro-phenolindophenol(4) to oxidize ascorbic acid before formation of the osazone is eliminated. The resulting method has been found to be quite satisfactory with respect to sensitivity, specificity, facility, and recovery of added ascorbic acid.
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