Abstract
Summary and Conclusion
Eastern equine encephalomyelitis virus has been propagated in a new host, Gambusia sp. embryos obtained from field specimens. A simple method for obtaining sterile embryonic material has been evolved. Standard in vitro conditions have been maintenance of embryos in 3-5 ml of 5 parts of Hanks' salt solution plus one part of ox serum-ultrafiltrate, at room temperature (23-25°C) in roller tubes rotating at 150-200 r.p.h. Virus potencies in individual cultures have varied from 10-3.7 to 10-6.3 by mouse intracerebral tests, through 8 culture generations. Multiple neutralization tests with the cultured virus have established the specific nature of the propagated agent. Latent mouse viruses in the embryonic material have been ruled out.
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