Survival of Tumor Homografts in Mice Pretreated with Ultrafiltrates of Hyaluronidase-Digested Tumor Supernatant. ∗

Abstract

Summary

Tumor extracts obtained by centrifugation, if incubated with hyaluronidase and recentrifuged, will pass readily through Pyrex glass ultrafme fritted discs and Selas #03 porcelain filters. They pass more slowly through a Berkefeld N filter, and least rapidly through a Seitz S-l asbestos filter, with over 50% loss of nitrogen-containing material. Filtrates from the first two filters, when injected into the prospective host prior to implanting a tumor homograft, are fully effective in abrogating the animal's normal resistance to the graft. Filtrates of the latter 2 filters show a slight effect, in terms of greater growth of the homografts prior to regression, as compared with uninjected controls.