Abstract
Conclusions
The method of Zlatkis, Zak, and Boyle for the direct determination of serum cholesterol has been adapted to the determination of total adrenal cholesterol. The values thus obtained do not differ significantly from those obtained by the method of Sperry and Webb in normal, hypophysectomized and cold stressed animals. The time required to analyze 10 samples and 5 standards by the Zlatkis, Zak? and Boyle procedure requires less than one hour compared to the 2 days usually necessary when methods based on the Liebermann-Burchard reaction are utilized.
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