Propagation of Bunyamwera,West Nile,Ilheus,and Br I Viruses in Human Cells in Tissue Culture. ∗

Abstract

Summary

1. Bunyamwera virus was grown in roller-slide tissue cultures of human lymph nodes (fibroblastic type cells). Propagation of virus was demonstrated by increases in virus titers in short-term virus growth studies, by long persistence of virus in tissue cultures as contrasted with rapid disappearance in cell-free preparations, and by persistence of virus after serial passages had diluted the original virus inoculum far beyond its minimal infective dilution. 2. Similar but less extensive studies demonstrated the propagation in human fibroblast tissue cultures of West Nile, Egypt 101, Ilheus, and Br I viruses. 3. Bunyamwera virus showed changes interpreted as an adaptation phenomenon during serial passages in tissue cultures. These changes were reversed by serial intracerebral passage in mice. 4. None of these viruses caused appreciable cytopathol-ogy under these experimental conditions, even after prolonged growth in tissue culture. 5. Bunyamwera, Egypt 101, and Ilheus viruses were also grown in tissue cultures from various human cancers. It was not possible to determine whether virus was propagating in the cancer cells, or in nonneoplastic stromal cells. 6. Egypt 101 virus propagated in cells from an immune patient, but not in the presence of immune serum.