Use of Tissue Culture Mediums Sterilized with Gamma Radiation from Cobalt-60. ∗

Abstract

Summary

Plasma, serum, and embryonic extract contaminated with gram negative and non-sporeforming gram positive bacteria have been shown to be sterilized by a dosage of 2 × 10⁵ rep of gamma radiation from Cobalt-60. Such treatment failed to alter the properties of these materials as related to their use in tissue culture work. These studies are being extended to cover dosages necessary to kill bacterial spores and efforts are being made to put the work on a more quantitative basis by utilizing established strains of tissue culture cells for assay procedures.