Abstract
Summary
1. A method is described for the determination of the respiratory rates of tissues in contact with oxygen by supporting them in the gas phase on fiber-glass mats in a modified wide-mouthed Warburg flask. 2. Investigated in this way the tissues maintained a satisfactory physiologic state as evidenced by slow decline in respiratory rate and negligible changes in pH. 3. For 8 rat tissues studied the respiratory rates on mats were considerably higher than those in Krebs-Ringer-Phosphate; and higher than those in Krebs Medium III in the case of muscle, brain, kidney, and heart, lower only for spleen, and not significantly different for liver, intestine, and testis. 4. Application of the respiratory rates to summated tissue calculations yielded the following values relative to BMR: on mats, 101.8%; in Krebs-Ringer-Phosphate, 78.7%; and in Krebs Medium III, 90.7%. 5. The utility of the technic using mats as a means of avoiding the variables due to different liquid media is discussed.
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