Abstract
Summary
1. A short simple method is given for obtaining from sheep pituitary glands a follicle-stimulating hormone preparation of high biological purity and low protein content. This method consists of extracting the glands with saturated sodium chloride solution at 72°C, adjusting the extract to pH 2.5, dialysis of the soluble fraction and recovery of the hormone from the dialyzed fraction by lyophilization or by the use of an anion exchanger. 2. When 5 g eq. were given to male and female hypophysectomized rats for 10 days, only a very small amount of luteinizing activity was found. The fraction insoluble at pH 2.5 contained both follicle-stimulating and luteinizing activities. 3. The results are discussed from the viewpoint that folliclestimulating hormone may exist in pituitary tissue in different forms.
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