Use of Hyla cinerea in Assay of Melanophorotropic Potency of ACTH

Abstract

Summary

In mice, a progressive lowering of blood nucleotide during pregnancy occurs simultaneously with an increase in nucleotide level for the embryos of such animals. Injection of adenylic acid into tumor-bearing mice produces a lowering of the nucleotide level in the tumor tissue, in contrast with no significant effect on the nucleotide level in embryo tissue. The liver and spleen undergo a lowering of tissue ATP as a function of pregnancy, and to a lesser extent as a function of tumor-growth. No significant alteration in muscle nucleotide is seen for mice bearing implanted tumors. The injection of adenylic acid elevates the ATP level in liver and spleen of normal, tumor-bearing, and pregnant animals, but, at least in the case of normal and tumor-bearing mice, has no effect on muscle nucleotide. Following adenylic acid injection, liver tissue from normal, tumor-bearing, and pregnant mice shows an increase in total purine, whereas the spleen does not.