Abstract
Summary
Bovine adrenal glands were perfused with blood containing added ACTH and the perfusates purified for study of their C19 steroid contents. Individual steroids present in the purified extracts were detected and characterized by studying the behavior of the free compounds and their derivatives by paper chromatographic techniques involving several solvent systems and color tests. By these methods, five 17-ketosteroids were found in adrenal perfusates, three of which exhibited properties identical to standard samples of Δ4-androstene-3,11,17-dione, Δ4-androstene-3, 11,17-trione and Δ4-androstene-11β-ol-3,17-dione. The conclusion is reached that the methods used here allow the identification of these three steroids in adrenal perfusates with a high degree of certainty.
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