Abstract
Summary
A method is described for the preparation of mitochondria from 50 to 100 g of liver. The tissue is pulped in a special press and homogenized at a reduced motor speed for a short time in the Waring blendor in isotonic sucrose. The mitochondria are isolated by differential centrifugation from isotonic sucrose. The ATP-ase, fatty acid oxidase, and succinoxidase activities of these preparations were shown to be similar to those of mitochondria prepared with a pestle type homogenizer.
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