Demonstration of β-Oxidation and Acetoacetate Formation in a Mouse Hepatoma. ∗

Abstract

Summary

Slices of a transplanted mouse hepatoma, C₉₅₄, oxidized octanoate-1-C¹⁴ to C¹⁴O₂ and acetoacetate-1, 3-C¹⁴. Isotopic ace-toacetate accumulated in quantities approaching that recovered in respiratory CO₂. The distribution of isotope in the ketone body is consistent with the view that the short-chain fatty acid was degraded by β-oxidation, the resulting C₂ fragments condensing to form ace-toacetate as well as being oxidized to CO₂.