Abstract
Summary
1. Monkey kidney epithelial cells can be cultured on glass without a plasma clot. The kidney fragments are induced to adhere to the wall of the test tube by preheating of the tube (45°C), and partial drying after introduction of tissue. These cultures compare well with cultures prepared in plasma clots, and respond as readily to the introduction of poliomyelitis virus. A great advantage is realized in the simplicity of preparation of these cultures, and their incubation in stationary racks. Exclusive of preliminary preparations and subsequent stoppering of the tubes, two trained workers can prepare well over 1000 cultures in 1 hour. About 2000 such cultures can be provided from a single monkey. 2. Bottle cultures can be prepared by the same technic, and have yielded poliomyelitis virus titers of about 10-6 per 0.1 ml inoculum. Titers in tubes or bottles reach their maximum in 24 to 72 hours depending upon the dose of virus inoculated. 3. After a culture has grown to satisfactory dimensions, original tissue fragments may be shaken off and transferred to new vessels. The fragments settle and produce comparable outgrowth in a shorter period of time than in the original culture.
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