Filter Paper Electrophoresis of Serum Proteins of the Domestic Fowl. ∗

Abstract

Summary

1. An albumin and 5 globulin fractions have been observed on electropherograms of sera of sexually immature pullets. The albumin fraction of such sera was associated with lipid, but lipid was not observed in association with the albumin of sera of estrogenized pullets. 2. Zones of the electropherograms of sera of pullets that stained with Oil Red O also gave the Feulgen plasmalogen reaction. The Feulgen reaction was stronger with sera of estrogenized birds. 3. Electropherograms of yolk dispersed in saline were prepared using 20% methanolic veronal buffer. A presumptive phosphoprotein fraction was distinguishable on these papers; it resembled closely the presumptive phosphoprotein fraction of sera of laying hens or estrogenized immature pullets. The presumptive phosphoprotein zones were doubled for both yolk and sera. 4. Studies of electropherograms of estrogenized immature pullets that had received injections of phosphate labeled with P³² showed that a large moiety of the phospholipid moved ahead of the phosphoprotein fraction when methanolic buffer had been used to separate this PP fraction, although a part of the phospholipid did remain in association with the PP fraction.