Abstract
Summary
No differences were observed between the staining intensities of spermatozoal nuclei in untreated and glycerol treated semen, either before freezing or after thawing, when they were stained at pH 4, 5, 6, 7, 8, 9, or 10 with certain oxidation-reduction potential indicator dyes. On the basis of this criterion, it has been demonstrated that treating 9 parts of liquefied semen with one part absolute glycerol, placing it in an insulated box containing dry ice (—70°C) for 24 hours, and then thawing it in 37°C water bath does not modify those proteins of the spermatozoal nucleus which are stained.
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