Excretion of Ether-Soluble Acids by Rats on Necrogenic Diet with and Without Supplements of Antibiotics. ∗

Abstract

Summary

1. Addition of aureomycin or penicillin to the necrogenic diet showed not effect on the quantity of nitrogen, urea, ammonia, creatinine, hippuric acid or para-aminobenzoic acid excreted in the urine by rate. 2 Most rats on necrogenic diet die of liver necrosis between 30 and 60 days; a few survive. In these rats a statistically significantly higher urinary excretion of either soluble acids was found than in rats treated with aureomycin or penicillin. Chromatograms of these ether-soluble acids indicated that addition of aureomycin or penicillin to the diet but not vitamin E altered the components of these acids in the urine. 3. The ether-soluble acids in the non-hydrolysed urine consists of para-aminobenzoic acid, hippuric acid, methyl malonic acid, α, α-dimethyl succinic acid and at least 2 unidentified acids. Methyl malonic acid forms a la0rge part of the total ether-soluble acids in the non-treated and vit. E-treated animal, and forms a smaller fraction of the acid excretion of the animals receiving penicillin or aureomycin.