Abstract
Summary
A method is described for the quantitative colorimetric estimation of vit. A and carotene in each other's presence. The method is based on the fact that the purple-red color developed by vit. A (maximum absorption 525-530 mμ) and the bluish-green color developed by carotene (maximum absorption 725-760 mμ) in the presence of perchloric acid, may be stabilized in a solution of amyl acetate. In a mixture of vit. A and carotene, carotene may be estimated directly by determining its absorption at 750 mμ the amounts of vit. A may be calculated by subtracting from the absorption, measured at 525 mμ the absorption value which corresponds to carotene at this wave length.
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