Intrinsic Factor Studies I. Paper Electrophoresis of Mixture of Gastric Juice and Radioactive Vitamin B 12 . ∗

Abstract

Summary

1. Results of paper electrophoresis of mixtures of radioactive vit. B₁₂ and concentrated human gastric juice demonstrate that the radioactive B12 is bound to a component of gastric juice which is not one of the major protein peaks and moves toward the anode. This behavior of the vit. B₁₂ was not noted when it was mixed with saline or serum. 2. This localization of vit. B₁₂ to a specific portion of gastric juice by paper electrophoresis may be a clue to the further identification of the intrinsic factor of Castle.