Abstract
Summary
When the isolated rat liver was perfused with a solution containing whole rat's blood diluted 1:3 with a solution of electrolytes, crystalline albumin, amino acids, and vitamins, bile was continuously formed over a period of 2 to 11 hours. The initial perfusate level of cholesterol averaged 13.8 mg %, and the initial perfusate level of cholate averaged 1.2 mg %. The final perfusate levels of cholesterol averaged 18.0 mg %, and of cholate 1.4 mg %. The differences between initial and final concentrations are not significant for these data. The bile contained an average of 6.3 mg % of cholesterol and 117 mg % of cholate. These data demonstrate that the isolated perfused rat liver maintains its ability to synthesize and degrade cholesterol.
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