Abstract
Summary
A simple, easily reproducible method for preparation of high-titer hemagglutinins with several arthropod-borne viruses is described. The method is based on the use of brains from newborn mice infected with virus as the source material, and on treatment with acetone and ether for the elimination of nonspecific factors. With this method, stable specific HA has been found associated with the following viruses: dengue (Hawaiian type), dengue (New Guinea B type), Ilhëus, Japanese B encephalitis, Ntaya, West Nile (Egypt 101 strain), and yellow fever (Asibi strain). As yet, no agglutination has been uncovered associated with Anopheles A, Eastern equine encephalitis, poliomyelitis (Type 2, MEF 1 strain), Russia Far Eastern encephalitis, and West Nile (original strain) viruses.
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