Abstract
Summary
The liver appears to be the main site of the conversion of pteroglyglutamic acid (PGA) to citrovorum factor (CF) in the body. Other tissues had very limited capacity to form CF, with the exception of bone marrow which, relative to the small amount present in this tissue, formed an appreciable quantity of the factor. The hepatic tissue of rats which had been depleted of folic acid and CF was flooded with CF within one hour following the intraperitoneal injection of PGA. Homogenates of rat or chick liver, when incubated with PGA under an atmosphere of nitrogen, were capable of forming CF. Little CF was found when similar preparations were incubated under oxygen. The yield of CF was consistently increased by the presence of ascorbate in the incubation mixture.
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