Abstract
Summary
The equipment and the procedures have been devised for quantitative complement fixation in volumes of 12 mm3. The technic, reduced to a routine basis, is illustrated in the results obtained in the titration of complement and in the estimation of complement fixed by pneumococcus polysaccharide type VIII and its specific antiserum induced in the rabbit. With the procedures described the reaction can be carried out with antigen and antiserum in amounts of the order of 100-fold less than those required for the volumes usually employed, greatly extending the range for the study of material available only in small quantities.
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