Observations on Preservation of Human Spermatozoa at Low Temperatures

Abstract

Summary

Initial experiments indicate that when 9 parts of liquefied human semen are treated with 1 part of absolute glycerol and placed in an insulated box containing dry ice, it can be stored for at least 3 months and, after quick thawing in a 37°C water bath, it will show an average spermatozoal survival of 67%, a figure superior to those realized with faster freezing methods. Such revived spermatozoa show no detectable alterations in motility or morphology.