Abstract
During the course of studies involving adrenal slice incubations. it was noted that extracts prepared from the slices after incubation sometimes contained 3 compounds which migrated very slowly on paper chromatograms. At other times these would not be present, or if present, in amounts which could not be detected.
The possibility was considered that these might represent conversion products of one of the known steroids. Consequently, hydrocortisone was carried out with 2.2 gg of adrenal cortical tissue slices. Four and seven tenths mg of hydrocortisone were added to the incubating medium which was saline-phosphate (4:1), pH 7.3. extracts made from the medium and slices were purified by silica gel chromatography. The purified fractions were then chromatographed on filter paper using the technic of Bush 1 . In this particular in cubation, adrenal slice incubated without added hydrocortisone produced no detectable quantities of the polar compounds, whereas the extracts from the incubation with added hydrocortisone showed the 3 polar compounds on the paper chromatograms (Fig. 1). The hydrocortisone had previously been shown to be chromatographically homogeneous.
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