Abstract
Summary
A method for culturing a single layer of monunuclear agranulocytes from rat peritoneal exudate is described. Two-week cultures of this type consist chiefly of a network of fibroblasts interpersed with numerous macrophages, and are suitable for the maintenance of obligate intracellular parasites (ex.: Trypanosoma cruzi, leishmaniform stage). They may conveniently be used for drug testing, opsonic studies, or other tests requiring the exposure of a single layer of cells, normal or parasitised, to a supernatant liquid phase.
Get full access to this article
View all access options for this article.