Use of “Granuloma Pouch” Technic in the Study of Antiphlogistic Corticoids ∗

Abstract

Summary

1. Using the “granuloma pouch” technic. it was possible to produce very large amounts oi inflammatory tissue and fluid (corresponding, in some cases, to as much as 25% of total body weight) in the rat, without interfering with the general well-being of the animal. 2. The introduction of small amounts of hydrocortisone, directly into the wall of the pouch, completely prevents the development of these inflammatory structures. 3. Introduction of the same amount of hydrocortisone into the lumen of the pouch, or into connective tissue at a distance of only about one mm from the pouch, is virtually ineffective. 4. The croton oil used to produce these granulonia pouches retained part of its irritating effects, even after a sojourn of 14 days, in hydrocortisone protected connective tissue. Although here it failed to cause inflammation, it still produced considerable damage, with hemorrhagic necrosis, when it was introduced into the unprotected connective tissue of other recipient animals.